Inhibition of Japanese encephalitis virus proliferation by long non-coding RNA SUSAJ1 in PK-15 cells

Abstract Background Japanese encephalitis virus is a mosquito-borne neurotropic flavivirus that causes acute viral in humans. Pigs are crucial amplifier host of JEV. Recently, increasing evidence has shown long non-coding RNAs (lncRNAs) play important roles infection. Methods JEV proliferation was evaluated after overexpression or knockdown lncRNA-SUSAJ1 using western blotting and reverse-transcription polymerase chain reaction (RT-PCR). C–C chemokine receptor type 1 (CCR1) found to regulate the expression by inhibitors screen. The detected RT-PCR transcription factor SP1. In addition, enrichments SP1 on promoter were analyzed chromatin immunoprecipitation. Results this study, we demonstrated swine could suppress PK-15 cells. We also CCR1 inhibited via upregulated lncRNA-SUSAJ1, resulting resistance proliferation. Conclusions These findings illustrate importance lncRNAs proliferation, reveal how regulates cells promote its